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  • Level 1 Asked on May 25, 2016 in Bacteriology.

    Gram positive bacilli. Please do the biochemical tests with it.  If the bacteria is any kind of practical use go for 16SrRNA sequencing to identify the bacteria.

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  • Level 1 Asked on December 8, 2015 in Molecular Biology.

    I don’t know whether I understood the question properly as you asked for.  Based on the question DNA polymerase have nucleotide binding pocket which allows them to differentiate between dNTP and NTP.

    RNA polymerase does not have  this pocket to differentiate between rNTP and dNTP. But closely looking at the structural model, both of these molecules does differ in size, i.e. size differentiation between rNTP and dNTP. Smaller molecule would not fit efficiently or rather be loose but if a larger molecule (rNTP) is allowed it would fit efficiently. Hence there is always we can find difference in processivity between DNA Pol and RNA Pol.

    Looking ahead for further explained answers by others. 🙂

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  • Level 1 Asked on December 8, 2015 in Molecular Biology.

    Protein expression is much related and dependent on gene expression. Although common to explain that in central dogma, DNA allows synthesizing RNA and then RNA to protein.

    Looking initially on genetic expression, genes are regulated by operator-promoter mechanism, repressor and other associated molecular factors.  For Eukaryotes you would find signal transduction mechanism where transcription factors are also responsible for expression. All these expressions  are ultimately meant to express proteins required by the cell or organism.

    RNA produced lead to expression of proteins. If you look at RNA particularly there are not all RNAs involved to associated with protein expression. So as you explained in your query it is right that all genetic expression does not always lead to protein expression.

    Protein expression depends upon the type of genes responsible and also the genetic machinery following its expression.

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  • Level 1 Asked on December 8, 2015 in Molecular Biology.

    Intrauterine infection may lead to infection to babies if the membrane gets ruptured. Cesarean birth cases ideally have more frequency of this infection since Serratia marcescens is transmitted hand to hand. If it is a common nosocomial infection then Hospitals take requisite measures.

    Looking ahead for more answers 🙂

    This answer accepted by Eva. on December 8, 2015 Earned 5 points.

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  • Level 1 Asked on December 7, 2015 in Bacteriology.

    If I say this it would be a debate. If we talk about the genetic make up of bacteria, they also contain extrachromosomal DNA often known to be plasmid. Plasmid can be transferred and hence an excellent phenomenon used by multiple bacteria to be risistant to certain antibiotics. Development of resistance develops due to mutation rate.

    Another phenomenon which is often seen in biofilms are called persister cells. Persister cells are dormant and do not grow. Normal antibiotics react to those whose metabolic process is active or actively growing cells. But for persisters they are dormant and hence antibiotics can not defeat their mechanism.

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  • Level 1 Asked on December 7, 2015 in Basic Microbiology.

    A pathogen can not rise in human or in organism or else it would be inherited as we have mitochondria. S. marcescens is associated to urinary tract infection. If we have have look at the growing conditions of the bacterium it is able to survive in temperature ranging between 5 to 40 degree Celsius with pH ranging between 5-9. This might be a reason for this being a prime reason to favor survival in multiple conditions.

    I am also looking forward for further discussions over this 🙂

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  • Level 1 Asked on December 7, 2015 in Virology.

    The question is extremely practical and good so thumbs up for that.

    Prion protein normally present in all individuals and animals. Prion or Prp has two distinct types PrpC and PrpSc. PrpC is normal structural protein present in organism body but when it gets disoriented into a PrpSc which is practically ineffective unused product.

    Prion is not having genetic material but rather a mass of misfolded protein aggregate. It does not replicate rather depend on normal Prion proteins which is the target of distorting them. Without normal prion proteins PrpSc does not able to function. There are several research still understanding the while bunch of mechanism underlying this disease. Prion disease are classified as Transmissible spongiform encepahopathies (TSE) due to range of neurological disease it causes.

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