An HIV-positive patient asks you if you can tell him the chances of him progressing to symptomatic AIDS. Which one of the following tests would be most useful?

An HIV-positive patient asks you if you can tell him the chances of him progressing to symptomatic AIDS. Which one of the following tests would be most useful?
a. CD4 lymphocyte count
b. HIV antibody test
c. HIV RT PCR
d. Neopterin
e. HIV p24 antigen

Sagar Aryal Admin Asked on October 23, 2015 in Virology.
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4 Answer(s)

c. RT PCR

Gaurab Karki Level 2 Answered on October 23, 2015.
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Ans-c

docvijeta Level 1 Answered on October 25, 2015.
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RT PCR

Shehzad Level 1 Answered on October 25, 2015.
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Answer: C (HIV RT PCR)
All test mentioned in options can be useful in determining the HIV viral infection, pathogenicity, host response.
The most useful among them especially in determining the chances of progression to symptomatic AIDS is RT PCR.
This is due to the advancement of technique which directly measure the viral RNA present in the blood plasma. Therefore the viral load present in the blood can be measured in terms of viral load or viral titer or viral burden and generally for HIV, a viral load of more than 750,000 copies per ml blood plasma significantly increase the chances of progression to AIDS within 5 years.

HIV RT PCR is a nucleic acid amplification test for HIV RNA in which Viral RNA is first isolated from the blood plasma and then converted into cDNA using an enzyme termed reverse transcriptase. The reverse transcriptase allows a single strand of RNA to be translated into a complementary strand of DNA. Once that reaction occurs, the routine PCR method can then be used to amplify the DNA.
Thank you.
Bivek Timalsina
Lecturer, Pokhara University

Bivek Timalsina Level 1 Answered on October 29, 2015.
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